CD27 Antikörper (AA 24-187)

Produktnummer ABIN7600813

Der Kaninchen Polyklonal Anti-CD27-Antikörper wurde für FACS, ELISA und IHC validiert. Er ist geeignet, CD27 in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für CD27 Antikörper (AA 24-187) (ABIN7600813)

Target: CD27 (CD27 Molecule (CD27))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD27 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-CD27 Antikörper

Bindungsspezifität: AA 24-187

Verwendungszweck: Anti-Cd27 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Cd27 Antibody Picoband® (ABIN7600813). Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Cd27 recombinant protein (Position: P24-F187).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: CD27 CD27 Molecule". 2. Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, Jacquot S, Schlossman SF (Jun 1997). "CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein". Proceedings of the National Academy of Sciences of the United States of America 94 (12): 6346-51. 3. Yamamoto H, Kishimoto T, Minamoto S (Nov 1998). "NF-kappaB activation in CD27 signaling: involvement of TNF receptor-associated factors in its signaling and identification of functional region of CD27". Journal of Immunology 161 (9): 4753-9.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu CD27

Target: CD27 (CD27 Molecule (CD27))

Andere Bezeichnung: Cd27

Hintergrund:

Synonyms: Angiopoietin-related protein 4,Angiopoietin-like protein 4,Hepatic fibrinogen/angiopoietin-related protein,HFARP,ANGPTL4,ARP4, HFARP, PGAR,PP1158, PSEC0166, UNQ171/PRO197,

Tissue Specificity: Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. .

Background: CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Molekulargewicht: 45 kDa

Gen-ID: 21940

UniProt: P41272

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints