Geminin Antikörper (AA 21-196)

Produktnummer ABIN7600598

Dieses Anti-Geminin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Geminin in WB, IHC, ELISA, IF, ICC und FACS. Geeignet für Human und Maus.

Kurzübersicht für Geminin Antikörper (AA 21-196) (ABIN7600598)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Geminin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-Geminin Antikörper

Bindungsspezifität: AA 21-196

Verwendungszweck: Anti-Geminin/GMNN Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Geminin/GMNN Antibody Picoband® (ABIN7600598). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Geminin/GMNN recombinant protein (Position: V21-E196).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bongers, E. M. H. F., Opitz, J. M., Fryer, A., Sarda, P., Hennekam, R. C. M., Hall, B. D., Superneau, D. W., Harbison, M., Poss, A., van Bokhoven, H., Hamel, B. C. J., Knoers, N. V. A. M. Meier-Gorlin syndrome: report of eight additional cases and review. Am. J. Med. Genet. 102: 115-124, 2001. 2. Burrage, L. C., Charng, W.-L., Eldomery, M. K., Willer, J. R., Davis, E. E., Lugtenberg, D., Zhu, W., Leduc, M. S., Akdemir, Z. C., Azamian, M., Zapata, G., Hernandez, P. P., and 18 others. De novo GMNN mutations cause autosomal-dominant primordial dwarfism associated with Meier-Gorlin syndrome. Am. J. Hum. Genet. 97: 904-913, 2015. 3. de Munnik, S. A., Bicknell, L. S., Aftimos, S., Al-Aama, J. Y., van Bever, Y., Bober, M. B., Clayton-Smith, J., Edrees, A. Y., Feingold, M., Fryer, A., van Hagen, J. M., Hennekam, R. C., and 22 others. Meier-Gorlin syndrome genotype-phenotype studies: 35 individuals with pre-replication complex gene mutations and 10 without molecular diagnosis. Europ. J. Hum. Genet. 20: 598-606, 2012.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Andere Bezeichnung: GMNN

Hintergrund:

Synonyms: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1, ATP-dependent helicase 1, hHEL1, SMARCAD1, KIAA1122

Tissue Specificity: Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin, fibroblasts, keratinocytes and esophagus.

Background: Geminin, DNA replication inhibitor, also known as GMNN, is a protein in humans encoded by the GMNN gene. This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.

Molekulargewicht: 25 kDa

Gen-ID: 51053

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication