14-3-3 Pan (AA 174-245) Antikörper

Produktnummer ABIN726710

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB, IF (p), IHC (p), FACS, IF (cc) und IHC (fro) validiert. Er ist geeignet, in Proben von Human, Maus, Ratte und Saccharomyces cerevisiae zu detektieren.

Kurzübersicht für 14-3-3 Pan (AA 174-245) Antikörper (ABIN726710)

Target: 14-3-3 Pan

Reaktivität: Human, Maus, Ratte, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails

Bindungsspezifität: AA 174-245

Kreuzreaktivität: Human, Maus, Ratte, Saccharomyces cerevisiae

Homologie: Sheep,Drosophila

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human 14-3-3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: 14-3-3 Pan

Andere Bezeichnung: 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon

Hintergrund:

Synonyms: HS1, GW128, YWHAA, KCIP-1, HEL-S-1, 14-3-3 protein beta/alpha, Protein 154, Protein kinase C inhibitor protein 1, YWHAB

Background: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.

Gen-ID: 7529

UniProt: P31946