SRP54 Antikörper (AA 2-433)

Produktnummer ABIN7600501

Der Kaninchen Polyklonal Anti-SRP54-Antikörper wurde für ELISA, WB, IF, FACS und ICC validiert. Er ist geeignet, SRP54 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für SRP54 Antikörper (AA 2-433) (ABIN7600501)

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SRP54 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-SRP54 Antikörper

Bindungsspezifität: AA 2-433

Verwendungszweck: Anti-SRP54 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SRP54 Antibody Picoband® (ABIN7600501). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SRP54 recombinant protein (Position: V2-K433).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bellanne-Chantelot, C., Schmaltz-Panneau, B., Marty, C., Fenneteau, O., Callebaut, I., Clauin, S., Docet, A., Damaj, G.-L., Leblanc, T., Pellier, I., Stoven, C., Souquere, S., and 26 others. Mutations in the SRP54 gene cause severe congenital neutropenia as well as Shwachman-Diamond-like syndrome. Blood 132: 1318-1331, 2018. 2. Carapito, R., Konantz, M., Paillard, C., Miao, Z., Pichot, A., Leduc, M. S., Yang, Y., Bergstrom, K. L., Mahoney, D. H., Shardy, D. L., Alsaleh, G., Naegely, L., and 38 others. Mutations in signal recognition particle SRP54 cause syndromic neutropenia with Shwachman-Diamond-like features. J. Clin. Invest. 127: 4090-4103, 2017. 3. Gowda, K., Chittenden, K., Zwieb, C. Binding site of the M-domain of human protein SRP54 determined by systematic site-ed mutagenesis of signal recognition particle RNA. Nucleic Acids Res. 25: 388-394, 1997.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SRP54

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Andere Bezeichnung: SRP54

Hintergrund:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP25, USP21

Tissue Specificity: Isoform USP25a is found in most adult and fetal tissues, expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.

Background: The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Molekulargewicht: 256 kDa

Gen-ID: 6729

UniProt: P61011

Pathways: SARS-CoV-2 Protein Interaktom