SRP54 Antikörper (AA 262-476)

Produktnummer ABIN968262

Produktdetails anti-SRP54 Antikörper

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Bindungsspezifität: AA 262-476

Reaktivität: Human, Maus, Ratte, Hund, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SRP54 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Hund, Ratte (Rattus), Maus, Frosch

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SRP54 aa. 262-476

Klon: 30-SRP54

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-SRP54 Antikörper (ABIN968262)

Rapiejko, Gilmore: "Empty site forms of the SRP54 and SR alpha GTPases mediate targeting of ribosome-nascent chain complexes to the endoplasmic reticulum." in: Cell, Vol. 89, Issue 5, pp. 703-13, (1997) (PubMed).

Traianedes, Findlay, Martin, Gillespie: "Modulation of the signal recognition particle 54-kDa subunit (SRP54) in rat preosteoblasts by the extracellular matrix." in: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 20891-4, (1995) (PubMed).

Details zu SRP54

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Andere Bezeichnung: SRP54 (SRP54 Produkte)

Synonyme: Srp54k antikoerper, zgc:55842 antikoerper, zgc:85713 antikoerper, SRP54 antikoerper, signal recognition particle 54 antikoerper, signal recognition particle protein antikoerper, signal recognition particle subunit Srp54 antikoerper, signal recognition particle antikoerper, family with sequence similarity 177 member A1 antikoerper, signal recognition particle 54kDa S homeolog antikoerper, signal recognition particle 54kDa antikoerper, SRP54 antikoerper, srp54 antikoerper, SSO_RS04840 antikoerper, MA_RS23935 antikoerper, AF_RS03170 antikoerper, PAB_RS02540 antikoerper, MSP_RS07670 antikoerper, RCI_RS04175 antikoerper, TGAM_RS09885 antikoerper, MTBMA_RS08340 antikoerper, FAM177A1 antikoerper, srp54.S antikoerper, Srp54 antikoerper

Hintergrund: Ribosomes that exist freely in the cytosol or those attached to the ER are intrinsically the same in their translational properties. ER-bound ribosomes are responsible for the production of secretory proteins and integral ER, Golgi, lysosomal, and plasma membrane spanning proteins. Such proteins contain signal sequences that direct their synthesis to the ER membrane. As the nascent polypeptide emerges from the ribosome, a signal recognition particle (SRP) binds to the signal sequence and serves to couple the ribosome to the protein-translocating machinery in the ER membrane. Although the SRP is a 325 kDa ribonucleoprotein, its 54 kDa subunit (SRP54) mediates interaction with, and targeting of, the nascent protein to the ER. Via its C-terminal M-domain, SRP54 associates with the nascent protein and inhibits its elongation. This complex binds to the SRP receptor on the ER, the ribosome is delivered to the translocation machinery, SRP is released, and elongation resumes. Targeting and insertion are tightly coupled to a GTPase cycle that involves SRP54 and SRP receptor. Although the mechanisms are unclear, release of SRP from the ER-bound complex requires GTP hydrolysis.

Molekulargewicht: 54 kDa

Pathways: SARS-CoV-2 Protein Interaktom