ALIX Antikörper (AA 2-330)

Produktnummer ABIN7600490

Der Maus Monoklonal anti-ALIX Antikörper (Klon 14D10) (ABIN7600490) detektiert spezifisch ALIX in WB, IHC, IF, FACS und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für ALIX Antikörper (AA 2-330) (ABIN7600490)

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ALIX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 14D10

Produktdetails anti-ALIX Antikörper

Bindungsspezifität: AA 2-330

Verwendungszweck: Anti-ALIX/PDCD6IP Antibody (monoclonal, 14D10)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ALIX/PDCD6IP Antibody (monoclonal, 14D10) (ABIN7600490). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PDCD6IP recombinant protein (Position: A2-D330). Human PDCD6IP shares?96.7% and 95.2% amino?acid? (aa)?sequence?identity?with mouse?and rat PDCD6IP, respectively.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ALIX

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Andere Bezeichnung: PDCD6IP

Hintergrund:

Synonyms: Programmed cell death 6-interacting protein, PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95, PDCD6IP, AIP1, ALIX, KIAA1375

Tissue Specificity: Heart, but also skeletal muscle, kidney, brain and mammary gland.

Background: Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 96-100 kDa

Gen-ID: 10015

Pathways: p53 Signalweg, EGFR Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Tube Formation