PNN Antikörper (AA 2-241)

Produktnummer ABIN7600477

Der Kaninchen Polyklonal Anti-PNN-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, PNN in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PNN Antikörper (AA 2-241) (ABIN7600477)

Target: PNN (Pinin, Desmosome Associated Protein (PNN))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PNN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-PNN Antikörper

Bindungsspezifität: AA 2-241

Verwendungszweck: Anti-PNN/DRSP Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PNN/DRSP Antibody Picoband® (ABIN7600477). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PNN/DRSP recombinant protein (Position: A2-L241).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brandner, J. M., Reidenbach, S., Franke, W. W. Evidence that 'pinin, reportedly a differentiation-specific desmosomal protein, is actually a widespread nuclear protein. Differentiation 62: 119-127, 1997. 2. Brandner, J. M., Reidenbach, S., Kuhn, C., Franke, W. W. Identification and characterization of a novel kind of nuclear protein occurring free in nucleoplasm and in ribonucleoprotein structures of the 'speckle' type. Europ. J. Cell Biol. 75: 295-308, 1998. 3. Degen, W. G. J., Agterbos, M. A., Muyrers, J. P. P., Bloemers, H. P. J., Swart, G. W. M. memA/DRS, a putative mediator of multiprotein complexes, is overexpressed in the metastasizing human melanoma cell lines BLM and MV3. Biochim. Biophys. Acta 1444: 384-394, 1999.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PNN

Target: PNN (Pinin, Desmosome Associated Protein (PNN))

Andere Bezeichnung: PNN

Hintergrund:

Synonyms: Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A

Tissue Specificity: Detected in peripheral blood monocytes and neutrophils (at protein level).

Background: Pinin is a protein that in humans is encoded by the PNN gene. By yeast 2-hybrid analysis of HeLa cells, followed by sequence analysis, it was found that epitope-tagged human PNN interacted with the serine/arginine (SR)-rich proteins SRP75 (SRSF4, 601940), SRM300 (SRRM2, 606032), and SRRP130 (PNISR, 616653). Truncation analysis revealed that the polyserine/RS domain of PNN and flanking sequences participated in binding to these SR proteins. The 4 proteins colocalized in the nucleus of HCE-T cells, and overexpression of any of these proteins affected the distribution of the others between nuclear speckles and nucleoplasm.

Molekulargewicht: 82 kDa

Gen-ID: 5411

UniProt: Q9H307