Choline Acetyltransferase Antikörper (AA 19-612)

Produktnummer ABIN7600421

Dieses Anti-Choline Acetyltransferase-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Choline Acetyltransferase in WB, IHC und ELISA. Geeignet für Maus und Ratte.

Kurzübersicht für Choline Acetyltransferase Antikörper (AA 19-612) (ABIN7600421)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-Choline Acetyltransferase Antikörper

Bindungsspezifität: AA 19-612

Verwendungszweck: Anti-Choline Acetyltransferase/Chat Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Choline Acetyltransferase/Chat Antibody Picoband® (ABIN7600421). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived rat Choline Acetyltransferase/Chat recombinant protein (Position: E19-D612).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL,
1. Cohen-Haguenauer, O., Brice, A., Berrard, S., Van Cong, N., Mallet, J., Frezal, J. Localization of the choline acetyltransferase (CHAT) gene to human chromosome 10. Genomics 6: 374-378, 1990. 2. Harold, D., Peirce, T., Moskvina, V., Myers, A., Jones, S., Hollingworth, P., Moore, P., Lovestone, S., Powell, J., Foy, C., Archer, N., Walter, S., and 11 others. Sequence variation in the CHAT locus shows no association with late-onset Alzheimer's disease. Hum. Genet. 113: 258-267, 2003. 3. Kraner, S, Laufenberg, I., Strassburg, H. M., Sieb, J. P., Steinlein, O. K. Congenital myasthenic syndrome with episodic apnea in patients homozygous for a CHAT missense mutation. Arch. Neurol. 60: 761-763, 2003.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: Chat

Hintergrund:

Synonyms: Choline O-acetyltransferase (EC:2.3.1.6); CHOACTase; ChAT; Choline acetylase; Chat

Background: Choline acetyltransferase  (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Molekulargewicht: 83 kDa

Gen-ID: 290567

UniProt: P32738

Pathways: Skeletal Muscle Fiber Development