IRF1 Antikörper (AA 170-315)

Produktnummer ABIN7600281

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch IRF1 in WB, IHC, ELISA, ICC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für IRF1 Antikörper (AA 170-315) (ABIN7600281)

Target: IRF1 (Interferon Regulatory Factor 1 (IRF1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-IRF1 Antikörper

Bindungsspezifität: AA 170-315

Verwendungszweck: Anti-IRF1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-IRF1 Antibody Picoband® (ABIN7600281). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human IRF1 recombinant protein (Position: M170-L315).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Harada, H., Willison, K., Sakakibara, J., Miyamoto, M., Fujita, T., Taniguchi, T. Absence of the type I IFN system in EC cells: transcriptional activator (IRF-1) and repressor (IRF-2) genes are developmentally regulated. Cell 63: 303-312, 1990. 2. Itoh, S., Harada, H., Nakamura, Y., White, R., Taniguchi, T. Assignment of the human interferon regulatory factor-1 (IRF1) gene to chromosome 5q23-q31. Genomics 10: 1097-1099, 1991. 3. Ko, J., Gendron-Fitzpatrick, A., Splitter, G. A. Susceptibility of IFN regulatory factor-1 and IFN consensus sequence binding protein-deficient mice to brucellosis. J. Immun. 168: 2433-2440, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu IRF1

Target: IRF1 (Interferon Regulatory Factor 1 (IRF1))

Andere Bezeichnung: IRF1

Hintergrund:

Synonyms: Interferon regulatory factor 1, IRF-1, IRF1

Tissue Specificity: Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.

Background: Interferon regulatory factor 1, also known as MAR is a protein that in humans is encoded by the IRF1 gene. The IRF1 gene is mapped to chromosome 5q31.1 by pulsed-field gel electrophoresis. IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.?

Molekulargewicht: 48-50 kDa

Gen-ID: 3659

UniProt: P10914

Pathways: Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Immune Effector Process, Hepatitis C, Autophagie