ITCH Antikörper (AA 17-358)

Produktnummer ABIN7600264

Der Kaninchen Polyklonal Anti-ITCH-Antikörper wurde für ELISA, WB, IHC, IF, FACS und ICC validiert. Er ist geeignet, ITCH in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ITCH Antikörper (AA 17-358) (ABIN7600264)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ITCH Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-ITCH Antikörper

Bindungsspezifität: AA 17-358

Verwendungszweck: Anti-ITCH/AIP4 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ITCH/AIP4 Antibody Picoband® (ABIN7600264). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ITCH/AIP4 recombinant protein (Position: K17-E358).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dorjbal B, Derse D, Lloyd P, Soheilian F, Nagashima K, Heidecker G. ""The role of ITCH protein in human T-cell leukemia virus type 1 release"". J Biol Chem, 2011 Sep 9. 2. Rossi M, Inoue S, Walewska R, Knight RA, Dyer MJ, Cohen GM, Melino G. ""Caspase cleavage of Itch in chronic lymphocytic leukemia cells"". Biochem Biophys Res Commun, 2009 Feb 13.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Andere Bezeichnung: ITCH

Hintergrund:

Synonyms: Receptor-type tyrosine-protein kinase FLT3, 2.7.10.1, FL cytokine receptor, Fetal liver kinase 2, FLK-2, Fms-like tyrosine kinase 3, FLT-3, Tyrosine-protein kinase receptor flk-2, CD135, Flt3, Flk-2, Flt-3,

Tissue Specificity: Hematopoietic stem and progenitor cell- enriched populations. Found in brain, placenta and testis.

Background: ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molekulargewicht: 103 kDa

Gen-ID: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events