CPN1 Antikörper (AA 158-208)

Produktnummer ABIN7600158

Der Kaninchen Polyklonal Anti-CPN1-Antikörper wurde für WB, ELISA, ICC, FACS und IF validiert. Er ist geeignet, CPN1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für CPN1 Antikörper (AA 158-208) (ABIN7600158)

Target: CPN1 (Carboxypeptidase N Subunit 1 (CPN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-CPN1 Antikörper

Bindungsspezifität: AA 158-208

Verwendungszweck: Anti-CPN1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-CPN1 Antibody Picoband® (ABIN7600158). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CPN1 recombinant protein (Position: D158-N208).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cao, H., Hegele, R. A. DNA polymorphism and mutations in CPN1, including the genomic basis of carboxypeptidase N deficiency. J. Hum. Genet. 48: 20-22, 2003. 2. Gebhard, W., Schube, M., Eulitz, M. cDNA cloning and complete primary structure of the small, active subunit of human carboxypeptidase N (kininase 1). Europ. J. Biochem. 178: 603-607, 1989. 3. Hegele, R. A. Personal Communication. London, Ontario, Canada 2/24/2003.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CPN1

Target: CPN1 (Carboxypeptidase N Subunit 1 (CPN1))

Andere Bezeichnung: CPN1

Hintergrund:

Synonyms: N-alpha-acetyltransferase 15, NatA auxiliary subunit,Gastric cancer antigen Ga19,N-terminal acetyltransferase,NMDA receptor-regulated protein 1,Protein tubedown-1,Tbdn100,NAA15,GA19, NARG1, NATH, TBDN100,

Tissue Specificity: Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. .

Background: Carboxypeptidase N catalytic chain is an enzyme that in humans is encoded by the CPN1 gene. Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits, this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency.

Molekulargewicht: 52 kDa

Gen-ID: 1369

UniProt: P15169

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process