UBE2O Antikörper (AA 150-1283)

Produktnummer ABIN7600121

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch UBE2O in WB, ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für UBE2O Antikörper (AA 150-1283) (ABIN7600121)

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE2O Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-UBE2O Antikörper

Bindungsspezifität: AA 150-1283

Verwendungszweck: Anti-UBE2O Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-UBE2O Antibody Picoband® (ABIN7600121). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human UBE2O recombinant protein (Position: R150-A1283).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mashtalir, N., Daou, S., Barbour, H., Sen, N. N., Gagnon, J., Hammond-Martel, I., Dar, H. H., Therrien, M., Affar, E. B. Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O. Molec. Cell 54: 392-406, 2014. 2. Nagase, T., Kikuno, R., Hattori, A., Kondo, Y., Okumura, K., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 347-355, 2000. 3. Nguyen, A. T., Prado, M. A., Schmidt, P. J., Sendamarai, A. K., Wilson-Grady, J. T., Min, M., Campagna, D. R., Tian, G., Shi, Y., Dederer, V., Kawan, M., Kuehnle, N., Paulo, J. A., Yao, Y., Weiss, M. J., Justice, M. J., Gygi, S. P., Fleming, M. D., Finley, D. UBE2O remodels the proteome during terminal erythroid differentiation. Science 357: eaan0218, 2017. Note: Electronic Article.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu UBE2O

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Andere Bezeichnung: UBE2O

Hintergrund:

Synonyms: Homer protein homolog 3,Homer-3,HOMER3,

Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: Ubiquitin-conjugating enzyme E2 O is a protein that in humans is encoded by the UBE2O gene. Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway, protein ubiquitination, and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.

Molekulargewicht: 200 kDa

Gen-ID: 63893

UniProt: Q9C0C9