CTNNA1 Antikörper (AA 143-292)

Produktnummer ABIN7600057

Dieses Anti-CTNNA1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CTNNA1 in WB, IHC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CTNNA1 Antikörper (AA 143-292) (ABIN7600057)

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CTNNA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: 10I2

Produktdetails anti-CTNNA1 Antikörper

Bindungsspezifität: AA 143-292

Verwendungszweck: Anti-alpha 1 Catenin/CTNNA1 Antibody Picoband® (monoclonal, 10I2)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-alpha 1 Catenin/CTNNA1 Antibody Picoband® (monoclonal, 10I2) (ABIN7600057). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292). Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Vasioukhin, V., Bauer, C., Degenstein, L., Wise, B., Fuchs, E. Hyperproliferation and defects in epithelial polarity upon conditional ablation of alpha-catenin in skin. Cell 104: 605-617, 2001. 2. Herrenknecht K, Ozawa M, Eckerskorn C, Lottspeich F, Lenter M, Kemler R (November 1991). "The uvomorulin-anchorage protein alpha catenin is a vinculin homologue". Proc Natl Acad Sci U S A 88 (20): 9156-60. 3. "Entrez Gene: CTNNA1 catenin (cadherin-associated protein), alpha 1, 102 kDa"

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Andere Bezeichnung: CTNNA1

Hintergrund:

Synonyms: alpha catenin antibody, alpha E catenin antibody, Alpha E-catenin antibody, alphaE catenin antibody, Cadherin associated protein 102 kDa antibody, Cadherin associated protein antibody, Cadherin-associated protein antibody, CAP 102 antibody, CAP102 antibody, Catenin (cadherin associated protein) alpha 1 102 kDa antibody, Catenin (cadherin associated protein), alpha 1, 102 kDa antibody, Catenin alpha 1 antibody, Catenin alpha-1 antibody, CTNA1_HUMAN antibody, CTNNA 1 antibody, CTNNA1 antibody, FLJ36832 antibody, FLJ52416 antibody, NY REN 13 antigen antibody, OTTHUMP00000224141 antibody, OTTHUMP00000224147 antibody, Renal carcinoma antigen NY REN 13 antibody, Renal carcinoma antigen NY-REN-13 antibody

Tissue Specificity: Expressed ubiquitously in normal tissues.

Background: CTNNA1, also known as Catenin alpha-1 or Catenin (cadherin-associated protein), alpha 1, is a protein that in humans is encoded by the CTNNA1 gene. It is mapped to 5q31.2. When surface epithelium CTNNA1 was ablated, hair follicle development was blocked and epidermal morphogenesis was dramatically affected, with defects in adherens junction formation, intercellular adhesion, and epithelial polarity. In vitro, CTNNA1 null keratinocytes were poorly contact inhibited and grew rapidly. These differences were not dependent upon intercellular adhesion and were in marked contrast to keratinocytes conditionally null for another essential intercellular adhesion protein, desmoplakin Knockout keratinocytes exhibited sustained activation of the Ras-MAPK cascade due to aberrations in growth factor responses. It is concluded that features of precancerous lesions often attributed to defects in cell cycle regulatory genes can be generated by compromising the function of CTNNA1.

Molekulargewicht: 100 kDa

Gen-ID: 1495

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location