EIF3A Antikörper (AA 13-477)

Produktnummer ABIN7599942

Der Kaninchen Polyklonal Anti-EIF3A-Antikörper wurde für WB, IHC, ELISA, IF und FACS validiert. Er ist geeignet, EIF3A in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für EIF3A Antikörper (AA 13-477) (ABIN7599942)

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-EIF3A Antikörper

Bindungsspezifität: AA 13-477

Verwendungszweck: Anti-EIF3A Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-EIF3A Antibody Picoband® (ABIN7599942). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human EIF3A recombinant protein (Position: K13-H477).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ensinger, C., Obrist, P., Mikuz, G., Merkx, G., Smeets, D., Banziger, R., Bachmann, F., Burger, M. Assignment of the p150 subunit of the eukaryotic initiation factor 3A gene (EIF3A) to human chromosome band 10q26 by in situ hybridisation. Cytogenet. Cell Genet. 83: 74-75, 1998. 2. Holz, M. K., Ballif, B. A., Gygi, S. P., Blenis, J. mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events. Cell 123: 569-580, 2005. 3. Johnson, K. R., Merrick, W. C., Zoll, W. L., Zhu, Y. Identification of cDNA clones for the large subunit of eukaryotic translation initiation factor 3: comparison of homologues from human, Nicotiana tabacum, Caenorhabditis elegans, and Saccharomyces cerevisiae. J. Biol. Chem. 272: 7106-7113, 1997.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu EIF3A

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Andere Bezeichnung: EIF3A

Hintergrund:

Synonyms: Acid-sensing ion channel 1, ASIC1,Amiloride-sensitive cation channel 2, neuronal, Brain sodium channel 2, BNaC2, ASIC1, ACCN2, BNAC2

Tissue Specificity: Expressed in most or all neurons.

Background: Eukaryotic translation initiation factor 3 subunit A (eIF3a) is a protein that in humans is encoded by the EIF3A gene. Enables RNA binding activity. Contributes to translation initiation factor activity. Involved in IRES-dependent viral translational initiation, formation of cytoplasmic translation initiation complex, and viral translational termination-reinitiation. Located in cytosol, nucleolus, and nucleoplasm. Part of eukaryotic translation initiation factor 3 complex.

Molekulargewicht: 180 kDa

Gen-ID: 8661

UniProt: Q14152

Pathways: Ribonucleoprotein Complex Subunit Organization