NCBP1 Antikörper (AA 121-790)

Produktnummer ABIN7599841

Dieser Anti-NCBP1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von NCBP1 in WB, ELISA, IHC, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für NCBP1 Antikörper (AA 121-790) (ABIN7599841)

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NCBP1 Antikörper

Bindungsspezifität: AA 121-790

Verwendungszweck: Anti-NCBP1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NCBP1 Antibody Picoband® (ABIN7599841). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NCBP1 recombinant protein (Position: Y121-A790).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chadwick, B. P., Obermayr, F., Frischauf, A.-M. Nuclear cap binding protein maps close to the xeroderma pigmentosum complementation group A (XPA) locus in human and mouse. Genomics 35: 632-633, 1996. 2. Ishigaki, Y., Li, X., Serin, G., Maquat, L. E. Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20. Cell 106: 607-617, 2001. 3. Izaurralde, E., Lewis, J., McGulian, M., Jankowska, E., Darzynkewicz, E., Mattaj, I. W. A nuclear cap binding protein complex involved in pre-mRNA splicing. Cell 78: 657-668, 1994.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NCBP1

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Andere Bezeichnung: NCBP1

Hintergrund:

Synonyms: BAG family molecular chaperone regulator 5,BAG-5,Bcl-2-associated athanogene 5,BAG5,KIAA0873,

Tissue Specificity: Expressed in all tissues examined with lower levels in brain and testis.

Background: The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.

Molekulargewicht: 92 kDa

Gen-ID: 4686

UniProt: Q09161

Pathways: Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process