ARNTL Antikörper (AA 116-447)

Produktnummer ABIN7599774

Der Kaninchen Polyklonal Anti-ARNTL-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, ARNTL in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für ARNTL Antikörper (AA 116-447) (ABIN7599774)

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARNTL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ARNTL Antikörper

Bindungsspezifität: AA 116-447

Verwendungszweck: Anti-BMAL1/ARNTL Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-BMAL1/ARNTL Antibody Picoband® (ABIN7599774). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BMAL1/ARNTL recombinant protein (Position: R116-E447).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Hatanaka F, Matsubara C, Myung J, Yoritaka T, Kamimura N, Tsutsumi S, Kanai A, Suzuki Y, Sassone-Corsi P, Aburatani H, Sugano S,Takumi T (Dec 2010). "Genome-wide profiling of the core clock protein BMAL1 targets reveals a strict relationship with metabolism". Molecular and Cellular Biology. 30 (24): 5636-5648. 2. Pappa KI, Gazouli M, Anastasiou E, Iliodromiti Z, Antsaklis A, Anagnou NP (Feb 2013). "The major circadian pacemaker ARNT-like protein-1 (BMAL1) is associated with susceptibility to gestational diabetes mellitus". Diabetes Research and Clinical Practice. 99 (2): 151-7. 3. Richards J, Diaz AN, Gumz ML (Oct 2014). "Clock genes in hypertension: novel insights from rodent models". Blood Pressure Monitoring. 19 (5): 249-54.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ARNTL

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Andere Bezeichnung: ARNTL

Hintergrund:

Synonyms: Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5, bHLHe5, Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3, ARNTL, BHLHE5, BMAL1, MOP3, PASD3

Tissue Specificity: Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.

Background: Aryl hydrocarbon receptor nuclear translocator-like protein 1 is protein that in humans is encoded by the ARNTL gene. The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 75 kDa

Gen-ID: 406

UniProt: O00327

Pathways: Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effekt