ERCC1 Antikörper (AA 106-291)

Produktnummer ABIN7599669

Dieses Anti-ERCC1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ERCC1 in WB, ELISA, IF, FACS und ICC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ERCC1 Antikörper (AA 106-291) (ABIN7599669)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERCC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-ERCC1 Antikörper

Bindungsspezifität: AA 106-291

Verwendungszweck: Anti-ERCC1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ERCC1 Antibody Picoband® (ABIN7599669). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ERCC1 recombinant protein (Position: R106-E291).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Imoto, K., Boyle, J., Oh, K., Khan, S., Ueda, T., Nadem, C., Slor, H., Orgal, S., Gadoth, N., Busch, D., Jaspers, N. G., Tamura, D., DiGiovanna, J. J., Kraemer, K. H. Patients with defects in the interacting nucleotide excision repair proteins ERCC1 or XPF show xeroderma pigmentosum with late onset severe neurological degeneration. (Abstract) J. Invest. Derm. 127: S92 only, 2007. 2. McCabe, K. M., Hemphill, A., Akkari, Y., Jakobs, P. M., Pauw, D., Olson, S. B., Moses, R. E., Grompe, M. ERCC1 is required for FANCD2 focus formation. Molec. Genet. Metab. 95: 66-73, 2008.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Andere Bezeichnung: ERCC1

Hintergrund:

Synonyms: DNA excision repair protein ERCC-1, ERCC1

Tissue Specificity: Expressed in fetal brain.

Background: DNA excision repair protein ERCC-1 is a protein that in humans is encoded by the ERCC1 gene. The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Molekulargewicht: 33 kDa

Gen-ID: 2067

UniProt: P07992

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response