YTHDC2 Antikörper (AA 105-1231)
Kurzübersicht für YTHDC2 Antikörper (AA 105-1231) (ABIN7599661)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 105-1231
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Verwendungszweck
- Anti-YTHDC2 Antibody Picoband®
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Kreuzreaktivität (Details)
- No cross-reactivity with other proteins.
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Produktmerkmale
- Anti-YTHDC2 Antibody Picoband® (ABIN7599661). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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Aufreinigung
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human YTHDC2 recombinant protein (Position: K105-K1231).
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Isotyp
- IgG
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anti-YTH Domain Containing 2 (YTHDC2) (AA 1131-1430) antibody
YTHDC2 Reaktivität: Human WB Wirt: Kaninchen Polyclonal unconjugated
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Applikationshinweise
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fanale, D., Iovanna, J. L., Calvo, E. L., Berthezene, P., Belleau, P., Dagorn, J. C., Bronte, G., Cicero, G., Bazan, V., Rolfo, C., Santini, D., Russo, A. Germline copy number variation in the YTHDC2 gene: does it have a role in finding a novel potential molecular target involved in pancreatic adenocarcinoma susceptibility? Expert Opin. Ther. Targets 18: 841-850, 2014. 2. Jain, D., Puno, M. R., Meydan, C., Lailler, N., Mason, C. E., Lima, C. D., Anderson, K. V., Keeney, S. ketu mutant mice uncover an essential meiotic function for the ancient RNA helicase YTHDC2. eLife 7: e30919, 2018. Note: Electronic Article. 3. Morohashi, K., Sahara, H., Watashi, K., Iwabata, K., Sunoki, T., Kuramochi, K., Takakusagi, K., Miyashita, H., Sato, N., Tanabe, A., Shimotohno, K., Kobayashi, S., Sakaguchi, K., Sugawara, F. Cyclosporin A associated helicase-like protein facilitates the association of hepatitis C virus RNA polymerase with its cellular cyclophilin B. PLoS One 6: e18285, 2011. Note: Electronic Article. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Konzentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- YTHDC2 (YTH Domain Containing 2 (YTHDC2))
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Andere Bezeichnung
- YTHDC2
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Hintergrund
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Synonyms: One cut domain family member 2, Hepatocyte nuclear factor 6-beta, HNF-6-beta, One cut homeobox 2, Transcription factor ONECUT-2, OC-2, ONECUT2, HNF6B
Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
Background: YTH domain containing 2 is a protein that in humans is encoded by the YTHDC2 gene. This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line.
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Molekulargewicht
- 175 kDa
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Gen-ID
- 64848
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UniProt
- Q9H6S0
Target
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