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YTHDC2 Antikörper (AA 105-1231)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch YTHDC2 in WB, ELISA, IF, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.
Produktnummer ABIN7599661

Kurzübersicht für YTHDC2 Antikörper (AA 105-1231) (ABIN7599661)

Target

YTHDC2 (YTH Domain Containing 2 (YTHDC2))

Reaktivität

  • 8
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 9
Kaninchen

Klonalität

  • 6
  • 3
Polyklonal

Konjugat

  • 9
Dieser YTHDC2 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 2
    • 1
    • 1
    • 1
    AA 105-1231

    Verwendungszweck

    Anti-YTHDC2 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-YTHDC2 Antibody Picoband® (ABIN7599661). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human YTHDC2 recombinant protein (Position: K105-K1231).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Fanale, D., Iovanna, J. L., Calvo, E. L., Berthezene, P., Belleau, P., Dagorn, J. C., Bronte, G., Cicero, G., Bazan, V., Rolfo, C., Santini, D., Russo, A. Germline copy number variation in the YTHDC2 gene: does it have a role in finding a novel potential molecular target involved in pancreatic adenocarcinoma susceptibility? Expert Opin. Ther. Targets 18: 841-850, 2014. 2. Jain, D., Puno, M. R., Meydan, C., Lailler, N., Mason, C. E., Lima, C. D., Anderson, K. V., Keeney, S. ketu mutant mice uncover an essential meiotic function for the ancient RNA helicase YTHDC2. eLife 7: e30919, 2018. Note: Electronic Article. 3. Morohashi, K., Sahara, H., Watashi, K., Iwabata, K., Sunoki, T., Kuramochi, K., Takakusagi, K., Miyashita, H., Sato, N., Tanabe, A., Shimotohno, K., Kobayashi, S., Sakaguchi, K., Sugawara, F. Cyclosporin A associated helicase-like protein facilitates the association of hepatitis C virus RNA polymerase with its cellular cyclophilin B. PLoS One 6: e18285, 2011. Note: Electronic Article.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    YTHDC2 (YTH Domain Containing 2 (YTHDC2))

    Andere Bezeichnung

    YTHDC2

    Hintergrund

    Synonyms: One cut domain family member 2, Hepatocyte nuclear factor 6-beta, HNF-6-beta, One cut homeobox 2, Transcription factor ONECUT-2, OC-2, ONECUT2, HNF6B

    Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

    Background: YTH domain containing 2 is a protein that in humans is encoded by the YTHDC2 gene. This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line.

    Molekulargewicht

    175 kDa

    Gen-ID

    64848

    UniProt

    Q9H6S0
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