PYCR1 Antikörper (AA 10-319)

Produktnummer ABIN7599596

Der Kaninchen Polyklonal Anti-PYCR1-Antikörper wurde für WB, ELISA, IHC, FACS, IF und ICC validiert. Er ist geeignet, PYCR1 in Proben von Human zu detektieren.

Kurzübersicht für PYCR1 Antikörper (AA 10-319) (ABIN7599596)

Target: PYCR1 (Pyrroline-5-Carboxylate Reductase 1 (PYCR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PYCR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-PYCR1 Antikörper

Bindungsspezifität: AA 10-319

Verwendungszweck: Anti-PYCR1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PYCR1 Antibody Picoband® (ABIN7599596). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PYCR1 recombinant protein (Position: Q10-D319).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Al-Gazali, L. I., Sztriha, L., Skaff, F., Haas, D. Gerodermia osteodysplastica and wrinkly skin syndrome: are they the same? Am. J. Med. Genet. 101: 213-220, 2001. 2. De Ingeniis, J., Ratnikov, B., Richardson, A. D., Scott, D. A., Aza-Blanc, P., De, S. K., Kazanov, M., Pellecchia, M., Ronai, Z., Osterman, A. L., Smith, J. W. Functional specialization in proline biosynthesis of melanoma. PLoS One 7: e45190, 2012. Note: Electronic Article. 3. Dougherty, K. M., Brandriss, M. C., Valle, D. Cloning human pyrroline-5-carboxylate reductase cDNA by complementation in Saccharomyces cerevisiae. J. Biol. Chem. 267: 871-875, 1992.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PYCR1

Target: PYCR1 (Pyrroline-5-Carboxylate Reductase 1 (PYCR1))

Andere Bezeichnung: PYCR1

Hintergrund:

Synonyms: Tricarboxylate transport protein, mitochondrial, Citrate transport protein, CTP, Solute carrier family 25 member 1, Tricarboxylate carrier protein, SLC25A1, SLC20A3

Tissue Specificity: Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Background: Pyrroline-5-carboxylate reductase 1, mitochondrial is an enzyme that in humans is encoded by the PYCR1 gene. This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 35 kDa

Gen-ID: 5831

UniProt: P32322