INSIG1 Antikörper (AA 1-87)

Produktnummer ABIN7599566

Der Kaninchen Polyklonal anti-INSIG1 Antikörper wird verwendet zum Nachweis von INSIG1 in Proben von Human. Er wurde validiert für WB, ELISA, FACS, IHC, IF und ICC.

Kurzübersicht für INSIG1 Antikörper (AA 1-87) (ABIN7599566)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser INSIG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-INSIG1 Antikörper

Bindungsspezifität: AA 1-87

Verwendungszweck: Anti-INSIG1 Antibody

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Anti-INSIG1 Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human INSIG1 recombinant protein (Position: M1-R87).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Andere Bezeichnung: INSIG1

Hintergrund:

Background: Insulin induced gene 1, also known as INSIG1, is a protein which in humans is encoded by the INSIG1 gene. This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.

Gene Full Name: insulin induced gene 1

Molekulargewicht: 66 kDa

Gen-ID: 3638

UniProt: O15503

Pathways: ER-Nucleus Signaling