NCF2 Antikörper (AA 1-526)

Produktnummer ABIN7599462

Dieses Anti-NCF2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NCF2 in WB, IHC, ELISA und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für NCF2 Antikörper (AA 1-526) (ABIN7599462)

Target: NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-NCF2 Antikörper

Bindungsspezifität: AA 1-526

Verwendungszweck: Anti-NOXA2/p67phox/NCF2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-NOXA2/p67phox/NCF2 Antibody Picoband® (ABIN7599462). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NOXA2/p67phox/NCF2 recombinant protein (Position: M1-V526).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Francke, U., Hsieh, C.-L., Foellmer, B. E., Lomax, K. J., Malech, H. L., Leto, T. L. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. 2. Noack, D., Rae, J., Cross, A. R., Munoz, J., Salmen, S., Mendoza, J. A., Rossi, N., Curnutte, J. T., Heyworth, P. G. Autosomal recessive chronic granulomatous disease caused by novel mutations in NCF-2, the gene encoding the p67-phox component of phagocyte NADPH oxidase. Hum. Genet. 105: 460-467, 1999. 3. Sancho-Shimizu, V., Malo, D. Sequencing, expression, and functional analyses support the candidacy of Ncf2 in susceptibility to Salmonella Typhimurium infection in wild-derived mice. J. Immun. 176: 6954-6961, 2006.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NCF2

Target: NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))

Andere Bezeichnung: NCF2

Hintergrund:

Synonyms: Syntenin-1, Melanoma differentiation-associated protein 9, MDA-9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, TACIP18, Scaffold protein Pbp1, Syndecan-binding protein 1, SDCBP, MDA9, SYCL

Tissue Specificity: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.

Background: This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 60 kDa

Gen-ID: 4688

UniProt: P19878