GSDMD Antikörper (AA 1-484)

Produktnummer ABIN7599431

Der Kaninchen Polyklonal Anti-GSDMD-Antikörper wurde für WB, ELISA, IHC, IF, ICC und FACS validiert. Er ist geeignet, GSDMD in Proben von Human zu detektieren.

Kurzübersicht für GSDMD Antikörper (AA 1-484) (ABIN7599431)

Target: GSDMD (Gasdermin D (GSDMD))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GSDMD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-GSDMD Antikörper

Bindungsspezifität: AA 1-484

Verwendungszweck: Anti-GSDMD Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GSDMD Antibody Picoband® (ABIN7599431). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GSDMD recombinant protein (Position: M1-H484).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ruhl, S., Shkarina, K., Demarco, B., Heilig, R., Santos, J. C., Broz, P. ESCRT-dependent membrane repair negatively regulates pyroptosis downstream of GSDMD activation. Science 362: 956-960, 2018. 2. Shi, J., Zhao, Y., Wang, K., Shi, X., Wang, Y., Huang, H., Zhuang, Y., Cai, T., Wang, F., Shao, F. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 526: 660-665, 2015. 3. Zhu, Q., Zheng, M., Balakrishnan, A., Karki, R., Kanneganti, T.-D. Gasdermin D promotes AIM2 inflammasome activation and is required for host protection against Francisella novicida. J. Immun. 201: 3662-3668, 2018.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu GSDMD

Target: GSDMD (Gasdermin D (GSDMD))

Andere Bezeichnung: GSDMD

Hintergrund:

Synonyms: gasdermin D, gasdermin-D

Tissue Specificity: Brain, placental, and gut tissues.

Background: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Molekulargewicht: 30, 53 kDa

Gen-ID: 79792

UniProt: P57764

Pathways: Inflammasome