ATG4C Antikörper (AA 1-457)

Produktnummer ABIN7599415

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ATG4C in WB, ELISA, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ATG4C Antikörper (AA 1-457) (ABIN7599415)

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG4C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-ATG4C Antikörper

Bindungsspezifität: AA 1-457

Verwendungszweck: Anti-ATG4C Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ATG4C Antibody Picoband® (ABIN7599415). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ATG4C recombinant protein (Position: M1-L457).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Agrotis, A., Pengo, N., Burden, J. J., Ketteler, R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15: 976-997, 2019. 2. Gross, M. B. Personal Communication. Baltimore, Md. 12/16/2020. 3. Li, M., Hou, Y., Wang, J., Chen, X., Shao, Z.-M., Yin, X.-M. Kinetics comparisons of mammalian Atg4 homologues indicates selective preferences toward diverse Atg8 substrates. J. Biol. Chem. 286: 7327-7338, 2011.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu ATG4C

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Andere Bezeichnung: ATG4C

Hintergrund:

Synonyms: Cationic amino acid transporter 3,CAT-3,CAT3,Cationic amino acid transporter y+,Solute carrier family 7 member 3,SLC7A3,ATRC3, CAT3,

Tissue Specificity: Highly expressed in thymus, uterus and testis. Detected at lower levels in brain, mammary gland, prostate, salivary gland and fetal spleen. In brain, highest expression in thalamus, hippocampus and amygdala. .

Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized.

Molekulargewicht: 52 kDa

Gen-ID: 84938

Pathways: Autophagie