ACTR3 Antikörper (AA 1-418)

Produktnummer ABIN7599368

Der Kaninchen Polyklonal Anti-ACTR3-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, ACTR3 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ACTR3 Antikörper (AA 1-418) (ABIN7599368)

Target: ACTR3 (ARP3 Actin-Related Protein 3 (ACTR3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ACTR3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-ACTR3 Antikörper

Bindungsspezifität: AA 1-418

Verwendungszweck: Anti-Arp3/ACTR3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Arp3/ACTR3 Antibody Picoband® (ABIN7599368). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Arp3/ACTR3 recombinant protein (Position: M1-S418).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Kim, J., Lee, J. E., Heynen-Genel, S., Suyama, E., Ono, K., Lee, K., Ideker, T., Aza-Blanc, P., Gleeson, J. G. Functional genomic screen for modulators of ciliogenesis and cilium length. Nature 464: 1048-1051, 2010. 2. Machesky, L. M., Reeves, E., Wientjes, F., Mattheyse, F. J., Grogan, A., Totty, N. F., Burlingame, A. L., Hsuan, J. J., Segal, A. W. Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins. Biochem. J. 328: 105-112, 1997. 3. Robinson, R. C., Turbedsky, K., Kaiser, D. A., Marchand, J.-B., Higgs, H. N., Choe, S., Pollard, T. D. Crystal structure of Arp2/3 complex. Science 294: 1679-1684, 2001.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ACTR3

Target: ACTR3 (ARP3 Actin-Related Protein 3 (ACTR3))

Andere Bezeichnung: ACTR3

Hintergrund:

Synonyms: Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K (+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42, MIG1

Tissue Specificity: Ubiquitously expressed.

Background: The specific function of this gene has not yet been determined, however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene.

Molekulargewicht: 47 kDa

Gen-ID: 10096

UniProt: P61158

Pathways: RTK Signalweg, Regulation of Actin Filament Polymerization