CRBN Antikörper (AA 1-378)

Produktnummer ABIN7599329

Der Kaninchen Polyklonal Anti-CRBN-Antikörper wurde für WB, ELISA, ICC, IF und FACS validiert. Er ist geeignet, CRBN in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für CRBN Antikörper (AA 1-378) (ABIN7599329)

Target: CRBN (Cereblon (CRBN))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CRBN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-CRBN Antikörper

Bindungsspezifität: AA 1-378

Verwendungszweck: Anti-CRBN Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-CRBN Antibody Picoband® (ABIN7599329). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CRBN recombinant protein (Position: M1-H378). Human CRBN shares 95% amino acid (aa) sequence identity with both mouse and rat CRBN.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Fischer, E. S., Bohm, K., Lydeard, J. R., Yang, H., Stadler, M. B., Cavadini, S., Nagel, J., Serluca, F., Acker, V., Lingaraju, G. M., Tichkule, R. B., Schebesta, M., and 9 others. Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide. Nature 512: 49-53, 2014. 2. Higgins, J. J., Hao, J., Kosofsky, B. E., Rajadhyaksha, A. M. Dysregulation of large-conductance Ca(2+)-activated K(+) channel expression in nonsyndromal mental retardation due to a cereblon p.R419X mutation. Neurogenetics 9: 219-223, 2008. 3. Higgins, J. J., Pucilowska, J., Lombardi, R. Q., Rooney, J. P. A mutation in a novel ATP-dependent Lon protease gene in a kindred with mild mental retardation. Neurology 63: 1927-1931, 2004.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu CRBN

Target: CRBN (Cereblon (CRBN))

Andere Bezeichnung: CRBN

Hintergrund:

Synonyms: CRBN, AD-006, Protein cereblon

Background: Cereblon is a protein that in humans is encoded by the CRBN gene. This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 51 kDa

Gen-ID: 51185