NEK7 Antikörper (AA 1-302)

Produktnummer ABIN7599245

Der Kaninchen Polyklonal Anti-NEK7-Antikörper wurde für WB, ELISA, IF, IP, ICC und FACS validiert. Er ist geeignet, NEK7 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NEK7 Antikörper (AA 1-302) (ABIN7599245)

Target: NEK7

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NEK7 Antikörper

Bindungsspezifität: AA 1-302

Verwendungszweck: Anti-NEK7 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-NEK7 Antibody Picoband® (ABIN7599245). Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human NEK7 recombinant protein (Position: M1-S302). Human NEK7 shares 97.7% and 98% amino acid (aa) sequence identity with mouse and rat NEK7, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 2-4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. He, Y., Zeng, M. Y., Yang, D., Motro, B., Nunez, G. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 530: 354-357, 2016. 2. Kimura, M., Okano, Y. Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3. Cytogenet. Cell Genet. 94: 33-38, 2001. 3. Sharif, H., Wang, L., Wang, W. L., Magupalli, V. G., Andreeva, L., Qiao, Q., Hauenstein, A. V., Wu, Z., Nunez, G., Mao, Y., Wu, H. Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome. Nature 570: 338-343, 2019.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NEK7

Target: NEK7

Andere Bezeichnung: NEK7

Hintergrund:

Synonyms: NEK7, Serine/threonine-protein kinase Nek7, EC 2.7.11.1, Never in mitosis A-related kinase 7, NimA-related protein kinase 7

Background: NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.

Molekulargewicht: 35 kDa

Gen-ID: 140609

Pathways: Inflammasome