NDUFA13 Antikörper (AA 1-144)

Produktnummer ABIN7598998

Der Kaninchen Polyklonal Anti-NDUFA13-Antikörper wurde für WB, ELISA, IHC, IF, ICC und FACS validiert. Er ist geeignet, NDUFA13 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für NDUFA13 Antikörper (AA 1-144) (ABIN7598998)

Target: NDUFA13 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 13 (NDUFA13))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NDUFA13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-NDUFA13 Antikörper

Bindungsspezifität: AA 1-144

Verwendungszweck: Anti-GRIM19/NDUFA13 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GRIM19/NDUFA13 Antibody Picoband® (ABIN7598998). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GRIM19/NDUFA13 recombinant protein (Position: M1-T144).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Angebault, C., Charif, M., Guegen, N., Piro-Megy, C., Mousson de Camaret, B., Procaccio, V., Guichet, P.-O., Hebrard, M., Manes, G., Leboucq, N., Rivier, F., Hamel, C. P., Lenaers, G., Roubertie, A. Mutation in NDUFA13/GRIM19 leads to early onset hypotonia, dyskinesia and sensorial deficiencies, and mitochondrial complex I instability. Hum. Molec. Genet. 24: 3948-3955, 2015. 2. Angell, J. E., Lindner, D. J., Shapiro, P. S., Hofmann, E. R., Kalvakolanu, D. V. Identification of GRIM-19, a novel cell death-regulatory gene induced by the interferon-beta and retinoic acid combination, using a genetic approach. J. Biol. Chem. 275: 33416-33426, 2000. 3. Barnich, N., Hisamatsu, T., Aguirre, J. E., Xavier, R., Reinecker, H.-C., Podolsky, D. K. GRIM-19 interacts with nucleotide oligomerization domain 2 and serves as downstream effector of anti-bacterial function in intestinal epithelial cells. J. Biol. Chem. 280: 19021-19026, 2005.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu NDUFA13

Target: NDUFA13 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 13 (NDUFA13))

Andere Bezeichnung: NDUFA13

Hintergrund:

Synonyms: Long-chain-fatty-acid--CoA ligase 3,6.2.1.3,Long-chain acyl-CoA synthetase 3,LACS 3,ACSL3,ACS3, FACL3, LACS3,

Tissue Specificity: Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Background: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 is an enzyme that in humans is encoded by the NDUFA13 gene. This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.

Molekulargewicht: 16 kDa, 17 kDa

Gen-ID: 51079

UniProt: Q9P0J0

Pathways: Protein targeting to Nucleus, Negative Regulation of intrinsic apoptotic Signaling