Neurotrophin 3 Antikörper (AA 89-103)

Produktnummer ABIN7581927

Dieses Anti-Neurotrophin 3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Neurotrophin 3 in WB, IHC und IF. Geeignet für Human.

Kurzübersicht für Neurotrophin 3 Antikörper (AA 89-103) (ABIN7581927)

Target: Neurotrophin 3 (NTF3)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Neurotrophin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Neurotrophin 3 Antikörper

Bindungsspezifität: AA 89-103

Verwendungszweck: A Rabbit Polyclonal Antibody to proNT-3

Sequenz: (C)DTELLRQQRR YNSPR

Spezifität: Pro-domain of the NT-3 protein

Kreuzreaktivität (Details): The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3.

Homologie: Rat,mouse - identical

Produktmerkmale: Highly specific antibody directed against an epitope of human pro. Anti-pro Antibody (ABIN7581927) can be used in western blot and immunohistochemistry applications. It has been designed to recognize pro from rat, mouse and human samples. The antibody does not cross reacts with mature , pro- and mature NGF or pro- and mature BDNF.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:300-1:600

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Andere Bezeichnung: NTF3

Hintergrund: Pro-Neurotrophin 3, proNTF3,Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins. These are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.Until recently, the functional role of the neurotrophin prodomain was thought to assist in the correct folding of the mature protein and the sorting of the neurotrophin into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions. Several studies implicate both proNGF and proBDNF in several physiological and pathological conditions.It remains to be established whether proNT-3 has different biological functions than the mature NT-3 neurotrophin.

Gen-ID: 4908

UniProt: P20783

Pathways: RTK Signalweg, Neurotrophin Signalübertragung