Neuregulin 1 Antikörper (Extracellular)

Produktnummer ABIN7581925

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Neuregulin 1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Neuregulin 1 Antikörper (Extracellular) (ABIN7581925)

Target: Neuregulin 1 (NRG1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Neuregulin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Neuregulin 1 Antikörper

Bindungsspezifität: AA 101-113, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Neuregulin-1 Type III

Sequenz: (C)DKIFEYDSPT HLD

Spezifität: Extracellular

Kreuzreaktivität (Details): This antibody will recognize Neuregulin-1 Type III. It will not recognize Neuregulin-1 Type I, Type II and Type IV.

Homologie: Rat,mouse - identical

Produktmerkmale: Anti-NRG1 Type III (extracellular) Antibody (ABIN7581925) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NRG1 Type III from rat, human and mouse samples. The antibody is specific for NRG1 Type III and does not recognize other Neuregulin-1 isoforms.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DKIFEYDSPTHLD, corresponding to amino acid residues 101-113 of human Neuregulin-1 Type III

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Neuregulin 1

Target: Neuregulin 1 (NRG1)

Andere Bezeichnung: NRG1

Hintergrund: Neuregulin-1 isoform 10, CRD-NRG, Sensory and motor neuron derived factor, SMDF,Neuregulin-1 (NRG1) is a member of a subfamily of the epidermal growth factor family and plays an important role in the development of the peripheral nervous system. Through alternative RNA splicing and promoter usage, the NRG1 gene encodes an entire family of more than 15 transmembrane and secreted isoforms. Neuregulin-1 acts as an important regulator in the survival of Schwann cell precursors, Schwann cell proliferation, motility, axon ensheathment and myelination.Neuregulin-1 can be divided into different according to the N-terminal sequence. Neuregulin-1 Type III, a transmembrane protein, contains an epidermal growth factor-like domain, which alone is adequate to bind and activate ErbB receptor tyrosine kinases. Type III also contains a cysteine-rich domain called "cysteine-rich domain-neuregulins," that requires a proteolytic cleavage for full activity1,2.Knockout studies show that type III is the key isoform required for schwann cell generation. These studies also confirmed that type III isoform is necessary for promoting myelination and to regulate myelin thickness1,2. Several different studies also showed that NRG1 Type III isoform is also an important regulator of cholinergic signaling. Neuregulin-1 Type III regulates the expression of AChR genes and targeting of nAChRs to the neuronal cell surface. This isoform in particular is a critical regulator of presynaptic targeting of α7-containing nAChRs3.

Gen-ID: 3084

UniProt: Q02297

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation