NPFFR2 Antikörper (Extracellular, Loop 2)

Produktnummer ABIN7886652

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NPFFR2 in WB, FACS und LCI. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für NPFFR2 Antikörper (Extracellular, Loop 2) (ABIN7886652)

Target: NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPFFR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-NPFFR2 Antikörper

Bindungsspezifität: AA 186-199, Extracellular, Loop 2

Verwendungszweck: A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 2 (extracellular)

Sequenz: (C)EEKYYRVRLS SHNK

Homologie: Rat - identical Human - 12 out of 14 amino acid residues identical

Produktmerkmale: Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (ABIN7581922) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize NPFFR2 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)EEKYYRVRLSSHNK, corresponding to amino acid residues 186 - 199 of mouse NPFFR2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NPFFR2

Target: NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))

Andere Bezeichnung: NPFFR2

Hintergrund:

Synonyms: NPFFR2, G-Protein Coupled Receptor 74, GPR74

Description: Neuropeptide FF receptor 2, NPFFR1, and also known as GPR74, is a member of the G protein-coupled receptor superfamily containing two subtypes, NPFFR1 and NPFFR2.1-2NPFFR1 and NPFFR2 are the high affinity receptors the endogenous neuropeptides NPFF and NPAF, also known as RFamide peptides, which are characterized by their Arg-Phe-NH2 motif at the C-terminal end.1-2NPFFR2 is expressed in both the central and peripheral nervous system, as well as in other organs such as heart, kidney and adipose tissue macrophages.1-4NPFFR2 has been extensively studied for its role in pain modulation. Mice over-expressing NPFFR2 showed more sensitivity to mechanical and thermal noxious stimuli. In addition, a role in the modulation of the development of opioid tolerance and the regulation of withdrawal symptoms, has been suggested.3,5NPFFR2 signaling has been implicated in the regulation of energy homeostasis, through the inhibition of food intake and the modulation of metabolic rate and energy expenditure.2,3,6 In this regard, it is interesting to note that, NPFFR2 has been identified in adipose tissue macrophages, cells that are involved in the development of obesity-induced metabolic diseases.7

Gen-ID: 104443

UniProt: Q924H0

Pathways: cAMP Metabolic Process