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NPFFR2 Antikörper (Extracellular Domain)

NPFF2 Reaktivität: Human, Pferd IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049116
  • Target Alle NPFFR2 (NPFF2) Antikörper anzeigen
    NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))
    Bindungsspezifität
    • 8
    • 8
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reaktivität
    • 33
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Pferd
    Wirt
    • 31
    • 2
    Kaninchen
    Klonalität
    • 33
    Polyklonal
    Konjugat
    • 20
    • 3
    • 3
    • 3
    • 2
    • 2
    Dieser NPFFR2 Antikörper ist unkonjugiert
    Applikation
    • 23
    • 18
    • 4
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human NPFFR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%) Monkey, Elephant, Rabbit, Pig (94%) Dog, Bovine (89%) Mouse, Rat, Panda, Bat (83%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 18 amino acid peptide from 3rd extracellular domain of human NPFFR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%), Monkey, Elephant, Rabbit, Pig (94%), Dog, Bovine (89%), Mouse, Rat, Panda, Bat (83%).

    Type of Immunogen: Synthetic peptide
  • Applikationshinweise
    Approved: IHC, IHC-P (16 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))
    Andere Bezeichnung
    NPFF2 / NPFFR2 (NPFF2 Produkte)
    Synonyme
    GPR74 antikoerper, HLWAR77 antikoerper, NPFF2 antikoerper, NPGPR antikoerper, Gpr74 antikoerper, HG31 antikoerper, Npff2 antikoerper, Npgpr antikoerper, neuropeptide FF receptor 2 antikoerper, NPFFR2 antikoerper, Npffr2 antikoerper
    Hintergrund
    Name/Gene ID: NPFFR2
    Subfamily: Neuropeptide
    Family: GPCR

    Synonyms: NPFFR2, G protein-coupled receptor 74, HLWAR77, G-protein coupled receptor 74, Neuropeptide FF receptor 2, Neuropeptide FF 2, NPFF2, Neuropeptide FF 2 receptor, NPGPR, GPR74, NPFF-R2, NPFF2 receptor, NPGP receptor
    Gen-ID
    10886
    Pathways
    cAMP Metabolic Process
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