ENPP1 Antikörper (Extracellular)

Produktnummer ABIN7581869

Der Kaninchen Polyklonal Anti-ENPP1-Antikörper wurde für WB, IHC, FACS und LCI validiert. Er ist geeignet, ENPP1 in Proben von Human zu detektieren.

Kurzübersicht für ENPP1 Antikörper (Extracellular) (ABIN7581869)

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ENPP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-ENPP1 Antikörper

Bindungsspezifität: AA 711-725, Extracellular

Verwendungszweck: A Rabbit Polyclonal antibody to ENPP1 (extracellular)

Sequenz: CLYQDFRIPL SPVHK

Spezifität: Extracellular, C-term.

Homologie: Mouse - 14 out of 15 amino acid residues identical, Rat- 13 out of 15 amino acid residues identical

Produktmerkmale: Anti-ENPP1 (extracellular) Antibody (ABIN7581869) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ENPP1 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CLYQDFRIPLSPVHK, corresponding to amino acid residues 711 - 725 of human ENPP1 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200-1:400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ENPP1

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Andere Bezeichnung: ENPP1 

Hintergrund: Ectonucleotide Pyrophosphatase/ Phosphodiesterase Family Member 1, Plasma-Cell Membrane Glycoprotein PC-1, CD203a, Membrane Component Chromosome 6 Surface Marker 1, M6S1,Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), also known as Plasma-Cell Membrane Glycoprotein (PC-1) and CD203a, is a member the ecto-enzyme ENPP family that includes seven members (ENPP1-ENPP7)1.ENPP1 is involved in purinergic signaling as it catalyzes the hydrolysis of ATP or GTP to AMP or GMP, while generating inorganic pyrophosphates (PPi)1,2. Inorganic pyrophosphates inhibit bone and cartilage mineralization, hence making ENPP1 a key regulator of bone and cartilage development and remodeling 2,3. Indeed, several hereditary mineralization or calcification-related disorders, have been linked to loss-of-function mutations of ENPP1, such as general arterial calcification of infancy (GACI) or Cole disease 3.Recently, an intriguing new role of ENPP1 as a central regulator of immunity and tumor proliferation came to light. It was shown that ENPP1 is the critical enzyme that degrades the stimulator of interferon genes (STING) ligand, cyclic GMP-AMP (cGAMP)4,5. cGAMP was first described as an intracellular second messenger, synthesized by the enzyme cyclic-GMP-AMP synthase (cGAS) in response to self and pathogenic double-stranded DNA in the cytosol. Intracellular signaling of cGAMP is carried through the STING pathway which then directs several antiviral and anticancer cellular response mechanisms 4,5.cGAMP can also be released to the extracellular space where it can be transported into cells by receptors like MERTK 6, P2X7 6, SLC19A1 7 and LRRC8A 8. ENPP1 is the only known ecto-enzyme that can inactivate extracellular cGAMP to GMP and AMP, and thus functions as an inhibitor of the anti-tumor response elicited by extracellular cGAMP 4,9.

Gen-ID: 5167

UniProt: P22413

Pathways: Regulation of Carbohydrate Metabolic Process