BDKRB1 Antikörper (Extracellular)

Produktnummer ABIN7581830

Der Kaninchen Polyklonal Anti-BDKRB1-Antikörper wurde für WB, FACS und LCI validiert. Er ist geeignet, BDKRB1 in Proben von Maus zu detektieren.

Kurzübersicht für BDKRB1 Antikörper (Extracellular) (ABIN7581830)

Target: BDKRB1 (Bradykinin Receptor B1 (BDKRB1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BDKRB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-BDKRB1 Antikörper

Bindungsspezifität: AA 202-214, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor

Sequenz: (C)HEAWHFVRMV ELN

Spezifität: Extracellular, 2nd loop.

Homologie: Rat - 12 out of 13 amino acid residues identical Not recommended for human samples

Produktmerkmale: Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody (ABIN7581830) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize B1 Bradykinin Receptor from mouse and rat samples. The antibody won't recognize human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HEAWHFVRMVELN, corresponding to amino acid residues 202 - 214 of mouse BDKRB1 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu BDKRB1

Target: BDKRB1 (Bradykinin Receptor B1 (BDKRB1))

Andere Bezeichnung: BDKRB1

Hintergrund: BK-1 receptor, BKRB1, B1R, BKR1,Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function.1Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). The desArg9-BK and Lys-desArg9-BK peptides activate BKRB1 while BK and Lys-BK operate by activating BKRB2.2 Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Expression of BKRB1is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. BKRB1 was long considered not to be expressed in healthy tissues. However, recent work has demonstrated a low level of expression of BKRB1 in the CNS of rodent and primates. In contrast, BKRB2 is constitutively expressed on various cell types.3 BKRB1 represents a potential therapeutic target for treatment of inflammatory disorders and cardiovascular diseases.

Gen-ID: 12061

UniProt: Q61125

Pathways: ACE Inhibitor Pathway