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Rekombinanter Myc Tag Antikörper (AA 408-439)

Name: Rekombinanter Myc Tag Antikörper (AA 408-439)
Brand: antibodies-online
SKU: ABIN7581767
Price: 558.26 EUR
Availability: InStock

Animal-Free Reaktivität: Tag WB, ELISA, IF, FACS Wirt: Kaninchen Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody

Quantitative immunodetection of a myc tagged protein using the automated Protein Simple Western Immunoassay system. (Rekombinanter Myc Tag Antikörper (AA 408-439))

Whole mount of 2 dpf transgenic zebrafish expressing in the CNS the myc-epitope as a fusion to a fluorescent protein stained with recombinant Hyper-Myc Mouse and fluorescently labelled secondary antiy-mouse Fc antibody (Rekombinanter Myc Tag Antikörper (AA 408-439))

Improvement of affinitiy and Fv-region thermal stability of Hyper-Myc compared to hybridoma antibody Myc1- 9E10 recognizing the myc-epitope (Rekombinanter Myc Tag Antikörper (AA 408-439))

BLI

ELISA

4 Abbildungen

Produktnummer ABIN7581767

Datenblatt (PDF)

Target Alle Myc Tag Produkte

Myc Tag

Antikörpertyp

Recombinant Antibody
Bindungsspezifität
24

21

12

8

5

3

2

1
AA 408-439
Reaktivität
34

5

3
Tag
Wirt
77

50

10

9

6

5

1

1

1
Kaninchen
Passende Sekundärantikörper

Expressionssystem

Phage display
Klonalität
90

58

10
Monoklonal
Konjugat
81

13

9

7

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Myc Tag Antikörper ist unkonjugiert
Applikation
121

63

49

44

35

34

20

19

15

11

11

6

6

5

4

4

4

3

2

2

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck

Rabbit Anti-c-myc Antibody, animal-free mAb

Spezifität

This is an antibody originating from 9E10 hybridoma clone. Improved via phage display technology. Affinity and thermal stability improved over original mouse IgG clone 9E10

Kreuzreaktivität (Details)

No known cross reactivity. No binding to native c-Myc protein.

Produktmerkmale

This antibody can be detected with anti-rabbit Fc secondary antibodies.

Aufreinigung

Protein A purification

Güteklasse

Animal-Free

Immunogen

No immunization, animal-free antibody development. Origin of original 9E10 clone: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc

Klon

TUN219-2C1 (9E10)

Isotyp

IgG
anti-Myc Tag antibody
WB, IP, ELISA, IF Wirt: Maus Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reaktivität: Human WB, IP, IHC (p), FACS Wirt: Maus Monoclonal 9E10 unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reaktivität: Human FACS Wirt: Maus Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reaktivität: Human WB, IP, IHC (p), FACS Wirt: Maus Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF Wirt: Maus Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Wirt: Maus Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Wirt: Maus Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB Wirt: Ziege Polyclonal unconjugated

anti-Myc Tag (AA 410-419) antibody
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal Myc-A7 unconjugated

anti-Myc Tag (AA 408-439) antibody
Reaktivität: Human WB, IP, IF, IHC (fro), IHC (p) Wirt: Maus Monoclonal 9E10 unconjugated Recombinant Antibody

Applikationshinweise

Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 mg/mL

Buffer

PBS, pH 7.4,

Lagerung

-20 °C
Target

Myc Tag

Andere Bezeichnung

c-myc tag (Myc Tag Produkte)

Substanzklasse

Tag

Hintergrund

Synthetic peptide of human c-Myc, aa408-439. The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti c-Myc Tag antibody. It shows a higher affinity and stability in direct comparison to hybridoma antibody 9E10 that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity.

Molekulargewicht

1.2 kDa