FLT1 Antikörper (AA 1162-1260)

Produktnummer ABIN725795

Der Kaninchen Polyklonal Anti-FLT1-Antikörper wurde für WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, FLT1 in Proben von Human, Ratte und Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für FLT1 Antikörper (AA 1162-1260) (ABIN725795)

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FLT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-FLT1 Antikörper

Bindungsspezifität: AA 1162-1260

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat VEGFR1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-FLT1 Antikörper (ABIN725795)

Liu, Zou, Li, Wang, Wang, Hao, Ke, Li: "RUNX3 modulates hypoxia-induced endothelial-to-mesenchymal transition of human cardiac microvascular endothelial cells." in: International journal of molecular medicine, Vol. 40, Issue 1, pp. 65-74, (2017) (PubMed).

Cheng, Xiang, Yang, Ma, Zhao: "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." in: Cell biochemistry and biophysics, (2015) (PubMed).

Liu, Wang, Wang, Wang, Xue: "Paeoniflorin attenuates A?1-42-induced inflammation and chemotaxis of microglia in vitro and inhibits NF-?B- and VEGF/Flt-1 signaling pathways." in: Brain research, Vol. 1618, pp. 149-58, (2015) (PubMed).

Wang, Hao, Shi, Pu, Wang: "Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage." in: Neural regeneration research, Vol. 7, Issue 8, pp. 595-600, (2015) (PubMed).

Details zu FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Andere Bezeichnung: VEGFR1

Hintergrund:

Synonyms: VEGFR-1, Vascular endothelial growth factor receptor 1, Fms-like tyrosine kinase 1, FLT-1, Tyrosine-protein kinase receptor FLT, Flt1, Vegfr1

Background: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity, may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity).

Gen-ID: 54251

UniProt: P53767

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals