Rekombinanter Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41) Antikörper

Produktnummer ABIN7566426

Produktdetails

Target: Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human Immunodeficiency Virus (HIV)

Wirt: Human

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Verwendungszweck: anti-gp41 (HIV), mAb (rec.) (A2)

Produktmerkmale:

Recombinant Antibody. Recognizes gp41 from HIV. Targets the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation. Applications: ELISA, FACS, IP. Clone: A2. Isotype: Human IgG1. Formulation: Liquid. In PBS. HIV-1 Env consists of two subunits: the surface-exposed glycoprotein gp120, which contains the receptor (CD4) and coreceptor (CCR5 or CXCR4) binding sites, and the transmembrane subunit gp41, which is critical for virus-cell membrane fusion. Sequential binding of HIV-1 to its receptor and coreceptor are the first steps critical for viral entry, whereby CD4 binding triggers a series of conformational changes in Env involving both gp120 and gp41. In gp120, CD4 binding induces rearrangement of the variable loops V1/V2 and formation of the bridging sheet, which allows for repositioning of the V3 loop to facilitate coreceptor binding. In gp41, CD4 binding triggers exposure of the buried N-terminal HR1 domain and its formation into a trimeric coiled-coil structure. Upon receptor and coreceptor binding, the fusion peptide in gp41 is inserted into the cell membrane and the HR1 and HR2 regions rearrange to form the six-helix bundle, which is critical for creation of the fusion pore to enable membrane fusion and efficient viral entry. The antibody clone A2 was found to specifically target the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation.

HIV-1 Env consists of two subunits: the surface-exposed glycoprotein gp120, which contains the receptor (CD4) and coreceptor (CCR5 or CXCR4) binding sites, and the transmembrane subunit gp41, which is critical for virus-cell membrane fusion. Sequential binding of HIV-1 to its receptor and coreceptor are the first steps critical for viral entry, whereby CD4 binding triggers a series of conformational changes in Env involving both gp120 and gp41. In gp120, CD4 binding induces rearrangement of the variable loops V1/V2 and formation of the bridging sheet, which allows for repositioning of the V3 loop to facilitate coreceptor binding. In gp41, CD4 binding triggers exposure of the buried N-terminal HR1 domain and its formation into a trimeric coiled-coil structure. Upon receptor and coreceptor binding, the fusion peptide in gp41 is inserted into the cell membrane and the HR1 and HR2 regions rearrange to form the six-helix bundle, which is critical for creation of the fusion pore to enable membrane fusion and efficient viral entry. The antibody clone A2 was found to specifically target the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation.

Aufreinigung: Puified

Reinheit: >95 % (SDS-PAGE)

Immunogen: Lectin-purified gp140 (from ADA.C1 strain).

Klon: A2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Stable for at least 1 year after receipt when stored at -20°C.

Stable for at least 1 week when stored at +4°C.

Antigendetails

Target: Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41)

Andere Bezeichnung: gp41

Substanzklasse: Viral Protein