GSDMD Antikörper

Produktnummer ABIN7566424

Dieser Meerschweinchen Polyklonal Antikörper erkennt spezifisch GSDMD in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für GSDMD Antikörper (ABIN7566424)

Target: GSDMD (Gasdermin D (GSDMD))

Reaktivität: Maus

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser GSDMD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-GSDMD Antikörper

Verwendungszweck: anti-Gasdermin D (mouse), pAb (IN110)

Produktmerkmale:

Polyclonal Antibody. Recognizes full-length and cleaved C-terminus domain of mouse gasdermin D. Does not cross-react with human gasdermin D. Source/Host: Guinea pig. Applications: ELISA, WB. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. Inflammasomes are multimeric protein complexes that comprise a sensor (e.g. NLRP3), an adaptor (ASC/Pycard) and the procaspase-1. An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs). The inflammasome platform leads to activation of caspase-1, which further induces maturation of interleukin-1beta and -18 (IL-1beta and IL-18) through proteolytic cleavage of pro-IL-1beta and pro-IL-18. Activated caspase-1, and also the recently characterized caspase-11 non-canonical inflammasome pathway, also cleave the intracellular gasdermin D, which leads to a particular form of inflammatory cell death called pyroptosis. The gasdermin family members contain N-terminal domains that are capable of forming membrane pores to induce cytolysis, whereas the C-terminal domains of gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or -11 cleavage of gasdermin D is required for regulation of pyroptosis: upon protease cleavage of the gasdermin N- and C-domain linker, the disruption of the intramolecular domain interaction in the presence of lipids releases the N-domain to assemble oligomeric membrane pores that trigger cell death. Gasdermin D seems to be a key effector in the LPS-induced lethal sepsis.

Inflammasomes are multimeric protein complexes that comprise a sensor (e.g. NLRP3), an adaptor (ASC/Pycard) and the procaspase-1. An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs). The inflammasome platform leads to activation of caspase-1, which further induces maturation of interleukin-1beta and -18 (IL-1beta and IL-18) through proteolytic cleavage of pro-IL-1beta and pro-IL-18. Activated caspase-1, and also the recently characterized caspase-11 non-canonical inflammasome pathway, also cleave the intracellular gasdermin D, which leads to a particular form of inflammatory cell death called pyroptosis. The gasdermin family members contain N-terminal domains that are capable of forming membrane pores to induce cytolysis, whereas the C-terminal domains of gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or -11 cleavage of gasdermin D is required for regulation of pyroptosis: upon protease cleavage of the gasdermin N- and C-domain linker, the disruption of the intramolecular domain interaction in the presence of lipids releases the N-domain to assemble oligomeric membrane pores that trigger cell death. Gasdermin D seems to be a key effector in the LPS-induced lethal sepsis.

Aufreinigung: Puified

Reinheit: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse gasdermin D (C-terminus).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

Stable for at least 1 year after receipt when stored at -20°C.

Details zu GSDMD

Target: GSDMD (Gasdermin D (GSDMD))

Andere Bezeichnung: Gasdermin D

UniProt: Q9D8T2

Pathways: Inflammasome