O3FAR1 Antikörper (N-Term)

Produktnummer ABIN7565972

Der Kaninchen Polyklonal Anti-O3FAR1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, O3FAR1 in Proben von Maus zu detektieren.

Kurzübersicht für O3FAR1 Antikörper (N-Term) (ABIN7565972)

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser O3FAR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-O3FAR1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Ffar4 Antibody

Kreuzreaktivität (Details): Anti-Ffar4 is directed against mouse Ffar4 at a n-terminal position.

Produktmerkmale: Synonyms: rabbit anti-Ffar4 antibody, Ffar-4, Ffar 4, free fatty acid receptor 4, omega-3 fatty acid receptor 1, G-protein coupled receptor 120, G-protein coupled receptor GT01, Gpr120, O3far1

Aufreinigung: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-Ffar4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-terminus portion of mouse Ffar4 protein.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Ffar4 Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~40-48 kDa in size corresponding to Ffar4 by western blotting in the appropriate cell lysate or extract. Tested using positive controls Raw 264.7 WCL p/n W10-001-369 and NIH/3T3 WCL p/n W10-000-358. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu O3FAR1

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Andere Bezeichnung: Ffar4

Hintergrund: Background: Anti-Ffar4 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Free Fatty Acid 4 receptor (Ffa4 receptor or GPR120), a rhodopsin-like G protein coupled receptor (GPCR) subfamily member, is a receptor that senses specific fatty acids such as omega-3 fatty acid in fish oil or the endogenous signaling lipid, PHASA. Ffa4 receptor is enriched in lung, colon and adipose tissue but is also detected in many other tissues and cells. The activation of Ffar4 has multiple effects, including but not limited to inhibition of inflammation, improving insulin sensitivity and adipogenesis, and regulating hormone secretion from the gastro-intestinal system and pancreatic islets. Therefore, approaches that regulate FFA4 receptor activity could be developed as promising anti-diabetic and anti-inflammation drugs. GPR120 is the only fatty acid receptor that can sense lipids in adipose tissue, mature adipocytes, CD11c+ macrophages, and RAW264.7 cells making this receptor of potential importance in the prevention and treatment of metabolic and inflammatory diseases.

Gen-ID: 107221

NCBI Accession: NP_861413

UniProt: Q7TMA4

Pathways: Hormone Transport