POLR2A/RPB1 Antikörper (N-Term)

Produktnummer ABIN7565953

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch POLR2A/RPB1 in WB, ELISA, ChIP und FM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für POLR2A/RPB1 Antikörper (N-Term) (ABIN7565953)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Produktdetails anti-POLR2A/RPB1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: POL II Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human POL II.

Produktmerkmale: Synonyms: RPO2, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RPB1, POLR2A,

Aufreinigung: This affinity purified antibody is directed against human POL II.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-POL II antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminal portion of human POL II conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-POL II antibody has been tested in ChIP and ELISA, and is likely suitable for western blotting and immunofluorescence assays. Positive controls used in ChIP were EGF stimulated NR4A1 5', NR4A1 3' and FOS 3'. The negative control was total rabbit IgG. ChIP Dilution: User Optimized Western Blot Dilution: 1:1000 ELISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Andere Bezeichnung: POLR2A

Hintergrund: Background: Anti-POL II antibody will detect DNA-directed RNA polymerase II subunit RPB1, an enzyme composed of 12 subunits, which initiates the transcription of RNA from DNA, synthesizing many functional non-coding RNAs as well as messenger RNA precursors. POL II encodes a core element of the transcription machinery. Malfunctioning of the Pol II gene can cause diseases such as Cockayne Syndrome B and La Crosse Encephalitis. Anti-POL II Antibody is useful for researchers interested in DNA damage and repair studies.

Gen-ID: 5430

NCBI Accession: NP_000928

UniProt: P24928

Pathways: Regulatorische RNA Pathways