POLR2A/RPB1 Antikörper (Internal Region)

Produktnummer ABIN6264342

Produktdetails anti-POLR2A/RPB1 Antikörper

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Spezifität: POLR2A Antibody detects endogenous levels of total POLR2A.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POLR2A, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Andere Bezeichnung: POLR2A (POLR2A Produkte)

Synonyme: NCU02103.1 antikoerper, 19.m02399 antikoerper, POLR2A antikoerper, 220kDa antikoerper, Rpb1 antikoerper, Rpo2-1 antikoerper, POLR2 antikoerper, POLRA antikoerper, RPB1 antikoerper, RPBh1 antikoerper, RPO2 antikoerper, RPOL2 antikoerper, RpIILS antikoerper, hRPB220 antikoerper, hsRPB1 antikoerper, DNA-directed RNA polymerase II largest subunit antikoerper, DNA-directed RNA polymerase II subunit RPB1 antikoerper, RNA polymerase II large subunit Rpb1 antikoerper, RNA polymerase II subunit A antikoerper, DNA-directed RNA polymerase II largest subunit, putative antikoerper, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antikoerper, polymerase (RNA) II (DNA directed) polypeptide A antikoerper, NCU02103 antikoerper, BBOV_I003360 antikoerper, EDI_169920 antikoerper, CMU_000800 antikoerper, SJAG_01713 antikoerper, MGYG_01527 antikoerper, POLR2A antikoerper, TP03_0185 antikoerper, EBI_22514 antikoerper, CGB_E5020W antikoerper, polr2a antikoerper, Polr2a antikoerper

Hintergrund:

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity).

Gene: POLR2A

Molekulargewicht: 270kDa

Gen-ID: 5430

UniProt: P24928

Pathways: Regulatorische RNA Pathways