HIST1H3A Antikörper (C-Term)

Produktnummer ABIN7565940

Dieses Anti-HIST1H3A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HIST1H3A in ELISA, WB und IHC. Geeignet für Human.

Kurzübersicht für HIST1H3A Antikörper (C-Term) (ABIN7565940)

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HIST1H3A Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-HIST1H3A Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Histone H3 Antibody

Kreuzreaktivität (Details): Anti-Histone H3 is directed against the human histone 3 protein.

Produktmerkmale: Synonyms: rabbit anti-histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody

Aufreinigung: The product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Histone H3 antibody has been tested for use in ELISA and western blot. This antibody is suitable for IHC. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5.0 μg/mL Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.98 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store H3 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Andere Bezeichnung: HIST1H3A

Hintergrund: Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.

Gen-ID: 8350

NCBI Accession: NP_003520

UniProt: P68431