SMAD2 Antikörper (pSer465, pSer467)

Produktnummer ABIN7565931

Dieses Anti-SMAD2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SMAD2 in WB und ELISA. Geeignet für Human.

Kurzübersicht für SMAD2 Antikörper (pSer465, pSer467) (ABIN7565931)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-SMAD2 Antikörper

Bindungsspezifität: pSer465, pSer467

Verwendungszweck: SMAD2 phospho S465/phospho S467 Antibody

Kreuzreaktivität (Details): Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues.

Produktmerkmale: Synonyms: rabbit anti-SMAD2 pS465pS467 antibody, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2 antibody, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: SMAD2 pS465 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-SMAD2 pS465 pS467 affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 52 kDa in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:1,000 - 1:3,000 ELISA Dilution: 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.94 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Andere Bezeichnung: SMAD2

Hintergrund: Background: Smad2 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). Smad2 (Mothers against decapentaplegic homolog 2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Anti-SMAD2 pS465 pS467 antibody is ideal for researchers interested in Cancer, Immunology and Nuclear Signaling research.

Gen-ID: 4087, 5174511

UniProt: Q15796

Pathways: Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus