CRISPR-Cas9 (C-Term) Antikörper (HRP)

Produktnummer ABIN7565919

Der HRP-konjugierte Kaninchen Polyklonal anti-CRISPR-Cas9 Antikörper (ABIN7565919) detektiert spezifisch CRISPR-Cas9 in WB, ELISA und FM. Dieser Antikörper reagiert spezifisch mit Proben aus Streptococcus pyogenes.

Kurzübersicht für CRISPR-Cas9 (C-Term) Antikörper (HRP) (ABIN7565919)

Target: CRISPR-Cas9

Reaktivität: Streptococcus pyogenes

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Produktdetails

Bindungsspezifität: C-Term

Verwendungszweck: Cas 9 Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.

Produktmerkmale: Synonyms: rabbit anti-Cas9 antibody peroxidase conjugation, HRP conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Cas9 Peroxidase Conjugated Antibody has been tested for use in ELISA and suitable in Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:25,000-1:30,000 IF Microscopy Dilution: 1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: CRISPR-Cas9

Andere Bezeichnung: cas9

Hintergrund: Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

Gen-ID: 901176

NCBI Accession: NP_269215

UniProt: Q99ZW2