CD31 Antikörper (C-Term)

Produktnummer ABIN7565916

Der Kaninchen Polyklonal anti-CD31 Antikörper (ABIN7565916) detektiert spezifisch CD31 in WB, IHC und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für CD31 Antikörper (C-Term) (ABIN7565916)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD31 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-CD31 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: CD31 Antibody

Kreuzreaktivität (Details): CD31 PECAM1 antibody is directed against rat CD31 protein.

Produktmerkmale: Synonyms: rabbit anti-CD31 Antibody, rabbit anti-PECAM-1 antibody, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31 Antibody, PECAM1 Antibody, CD31/PECAM1 Antibody

Aufreinigung: The product is delipidated and defibrinated antiserum.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: CD31 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to CD31 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Immunohistochemistry Dilution: 4 μg/mL Western Blot Dilution: 1:500 ELISA Dilution: 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: Pecam1

Hintergrund: Background: Anti-CD31 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). CD31 (PECAM-1) is a 130 kDa platelet endothelial cell adhesion molecule encoded by the PECAM1 gene found on chromosome 17 in humans. CD31 is expressed on platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 plays a key role in modulation of integrin-mediated cell adhesion, leukocyte transendothelial migration, angiogenesis, apoptosis and macrophage phagocytosis. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. Anti-CD31 is ideal for researchers interested in Stem Cell Research, Epigenetics, and Cell-cycle Regulation research.

Gen-ID: 29583

NCBI Accession: NP_113779

UniProt: Q3SWT0

Pathways: Regulation of Actin Filament Polymerization