SOD1 Antikörper (HRP)

Produktnummer ABIN7565914

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SOD1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Rind (Kuh).

Kurzübersicht für SOD1 Antikörper (HRP) (ABIN7565914)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-SOD1 Antikörper (HRP)

Verwendungszweck: Superoxide Dismutase Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes].

Produktmerkmale: Synonyms: rabbit anti-Superoxide Dismutase 1 Antibody HRP conjugation, peroxidase conjugated rabbit anti-SOD1 antibody, peroxidase conjugated rabbit anti-Superoxide Dismutase 1 Antibody, rabbit anti-SOD1 antibody HRP conjugation, ALS 1 antibody, ALS antibody, ALS1 antibody, Amyotrophic lateral sclerosis 1 adult antibody, Amyotrophic lateral sclerosis 1 antibody, Cu/Zn SOD antibody, Cu/Zn superoxide dismutase antibody, Homodimer antibody, SOD 1

Aufreinigung: Superoxide Dismutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Superoxide Dismutase [Bovine erythrocytes]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Superoxide Dismutase Peroxidase Conjugated Antibody has been tested by Western blot and is suitable to be assayed against 1.0 μg of Superoxide Dismutase [Bovine erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500- 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: SOD1

Hintergrund: Background: Superoxide Dismutase destroys radicals which are normally produced within the cells and which are toxic to biological systems. They are an important antioxidant defense in nearly all cells exposed to oxygen. Mutations in the Superoxide Dismutase enzyme can cause ALS, A4V, G93A, Down Syndrome, ARDS, and COPD.

Gen-ID: 281495

UniProt: P00442

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis