Rekombinanter ErbB2/Her2 Antikörper

Produktnummer ABIN7565889

Der Humanized Monoklonal anti-ErbB2/Her2 Antikörper (Klon Abz518) (ABIN7565889) detektiert spezifisch ErbB2/Her2 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter ErbB2/Her2 Antikörper (ABIN7565889)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Antikörpertyp: Recombinant Antibody

Fragment: Fab fragment

Reaktivität: Human

Wirt: Humanized

Klonalität: Monoklonal

Konjugat: Dieser ErbB2/Her2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Klon: Abz518

Produktdetails anti-ErbB2/Her2 Antikörper

Verwendungszweck: Recombinant Anti-HER2 Fab Antibody

Produktmerkmale: Synonyms: CD340, cluster of differentiation 340, ERBB2 (human), human epidermal growth factor receptor 2, HER2/neu, recombinant Monoclonal HER2, RabMAb HER2, Trastuzumab, Herceptin, Humanized Antibody, Recombinant Anti-HER2 Fab antibody, HER-2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, p185erbB2

Aufreinigung: Humanized Recombinant HER2 Fab fragment was expressed in E.coli cells.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Recombinant Protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Application Note: Humanized Recombinant Anti-HER2 Fab fragment Antibody has been tested for use in Flow Cytometry, Western Blot, and ELISA. This antibody recognizes structured HER2 and will work in western blot when the protein has not been denatured with DTT or bMe. Although not tested, this antibody could be useful in in IHC and in in-vivo and other cellular assays. Specific conditions for reactivity should be optimized by the end user. Flow Cytometry Dilution: 1:100 Western Blot Dilution: 1:2,000 ELISA Dilution: 1:10,000 to 1:100,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.59 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 2-8° C. Dilute only prior to immediate use.

Haltbarkeit: 6 months

Details zu ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Andere Bezeichnung: ERBB2

Hintergrund: Background: HER2 (human epidermal growth factor receptor 2) is a member of the epidermal growth factor receptor (HER/EGFR/ERBB) family. It is also called ERBB2, CD340 or proto-oncogene Neu. The protein is a receptor tyrosine kinase located on the plasma membrane of cells. Activation of the tyrosine kinase promotes cell proliferation and suppresses apoptosis. HER2 can dimerize with any other member of the ErbB family, which results in auto phosphorylation of tyrosine residues and activation of signal pathways. HER2 is over expressed in a significant proportion of cancer patients, resulting in an increased disease recurrence and poor prognosis. Therefore an early diagnosis of the HER2 status of the tumors is necessary to decide the appropriate treatment. Besides in breast cancer HER2 is over expressed in variety of different tumors. The over expression of HER2 makes it a target for immunotherapy of certain tumor patients. The antibody Trastuzumab (Herceptin) which recognizes HER2 was approved for the treatment of HER2 positive breast cancer patients in 1998. The original approval was for treatment of HER2 positive metastatic breast tumors. Later studies have shown that Herceptin is also beneficial for early stage HER2 positive tumor patients and it is approved as an adjuvant treatment. Additionally the antibody is now approved also for other HER2 positive tumors (metastatic gastric adenocarcinoma). This antibody is made from a humanized Fab fragment making it specific, efficient, and effective. Humanized Recombinant Anti-HER2 Fab fragment Antibody is useful for researchers interested in Cancer research.

Gen-ID: 2064

NCBI Accession: NP_001005862

UniProt: P04626

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development