TUBA1B Antikörper (C-Term)

Produktnummer ABIN7565852

Der Maus Monoklonal Anti-TUBA1B-Antikörper wurde für WB, ELISA, IHC, IF, FM und MA validiert. Er ist geeignet, TUBA1B in Proben von Human zu detektieren.

Kurzübersicht für TUBA1B Antikörper (C-Term) (ABIN7565852)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TUBA1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Klon: 17H11-F10

Produktdetails anti-TUBA1B Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Alpha-Tubulin Antibody

Kreuzreaktivität (Details): This Loading Control antibody is directed against alpha tubulin.

Produktmerkmale: Synonyms: mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control

Aufreinigung: Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts. Immunohistochemistry Dilution: 2.5 μg/mL Western Blot Dilution: 1:1,000 ELISA Dilution: 1:300,000 IF Microscopy Dilution: 0.1 μg/mL Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Andere Bezeichnung: TUBA1B

Hintergrund: Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

Gen-ID: 17986283

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase