Protein A Antikörper (FITC)

Produktnummer ABIN7565825

Der Ziege Polyklonal Anti-Protein A-Antikörper wurde für WB, DB und FACS validiert. Er ist geeignet, Protein A in Proben von Staphylococcus aureus zu detektieren.

Kurzübersicht für Protein A Antikörper (FITC) (ABIN7565825)

Target: Protein A

Reaktivität: Staphylococcus aureus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Protein A Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Dot Blot (DB), Flow Cytometry (FACS)

Produktdetails anti-Protein A Antikörper (FITC)

Verwendungszweck: Protein A Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, and purified and partially purified Protein A.

Produktmerkmale: Synonyms: goat anti-Protein A Antibody, FITC Conjugated goat anti-Protein A Antibody, Protein A Fluorescein, Protein A FITC Antibody

Aufreinigung: Anti-Protein A Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Protein A (Staphylococcus aureus)

Immunogen Type: Native Protein

Labeling Ratio: 4.3

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Protein A has been tested in dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Protein A

Target: Protein A

Hintergrund: Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.