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Protein A Antikörper

Reaktivität: Staphylococcus aureus ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN739261
  • Target Alle Protein A Antikörper anzeigen
    Protein A
    Reaktivität
    • 49
    • 5
    • 1
    Staphylococcus aureus
    Wirt
    • 19
    • 15
    • 14
    • 5
    • 2
    Kaninchen
    Klonalität
    • 48
    • 2
    Polyklonal
    Konjugat
    • 9
    • 9
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Protein A Antikörper ist unkonjugiert
    Applikation
    • 33
    • 33
    • 12
    • 8
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Kreuzreaktivität
    Bakterien
    Kreuzreaktivität (Details)
    S. aureus
    Aufreinigung
    Purified by Protein A.
    Immunogen
    Recombinant protein A
    Isotyp
    IgG
    Top Product
    Discover our top product Protein A Primärantikörper
  • Applikationshinweise
    WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    Protein A
    Abstract
    Protein A Produkte
    Synonyme
    alpha protein antikoerper, repB antikoerper
    Hintergrund

    Synonyms: Immunoglobulin G binding protein A, Staphylococcal protein A, SPA, gG-binding protein A, Staphylococcus aureus.

    Background: Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.

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