Protein A Antikörper (HRP)

Produktnummer ABIN7565824

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Protein A in ELISA, WB und IHC. Er zeigt eine Reaktivität gegenüber Staphylococcus aureus.

Kurzübersicht für Protein A Antikörper (HRP) (ABIN7565824)

Target: Protein A

Reaktivität: Staphylococcus aureus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Protein A Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Protein A Antikörper (HRP)

Verwendungszweck: Protein A Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].

Produktmerkmale: Synonyms: goat anti-Protein A Antibody, HRP Conjugated goat anti-Protein A Antibody, Protein A Peroxidase, Protein A HRP Antibody

Aufreinigung: Anti-Protein A (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Protein A [Staphylococcus aureus]

Immunogen Type: Native Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Protein A Peroxidase Conjugated has been tested by ELISA and western blot and is assayed against 1.0 μg of Protein A [Staphylococcus aureus] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 is suggested for this product. Immunohistochemistry Dilution: 1:250 Western Blot Dilution: 1:500-1:2,000 ELISA Dilution: 1:2,000-1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Protein A

Target: Protein A

Hintergrund: Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins. Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Protein A (GOAT) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology.